Biological membranes, composed of complex assemblies of lipids and proteins, serve as physi-cal barriers in the cell and are sites for many cellular signaling events….

Integral membrane proteins (IMPs) play crucial roles in many aspects of biology by mediating the transfer of material and sig-nals between cells and their environment….

Neopentyl Glycol (NG) class detergents are revolutionary new amphiphiles which have already shown great utility in membrane protein studies. NG class detergents are a more…

Amphipol A8-35  Amphipathic surfactant for maintaining solubilized membrane proteins in detergent-free solutions. Serve as stabilizers of membrane proteins in aqueous solutions. Amphipols can substitute out…

Membrane protein studies have advanced significantly over thepast few years. This is partly due to advances in tools and reagentsused to manipulate this class of…

Detergent Properties Listed Alphabetically The detergents are arranged in alphabetical order. The conditions used to measure CMC valuesand aggregation numbers are located in the Alphabetial…

Chemical structure of Detergents used in biotechnology experiments. useful reference for understanding chemical interactions and structures through laboratory.

1- First Step! Look up other purifications from similar sources and similar membranes.Different tissues, organisms and membrane types sometimes require differ-ent detergents and conditions. e.g….

Abstract: Endotoxins contaminate proteins that are produced inE. coli.High levels of endotoxins can influence cellular assays and cause severe adverse effects when administered to humans….

Introduction Interleukin 21 (IL-21) is a recently discovered type I cytokine and classified in the family, which includes IL-2, IL-4, IL-7, IL-9, IL-13 andIL-15, all…

Abstract: Endotoxins are frequent contaminants of recombinant proteins produced inEscherichia coli. Due to their adverse effects, endotoxins have to be removed from recombinant proteins prior…

Introduction: Affinity sorbents and detoxification strategies are described to remove different amounts of endotoxin. Advantages and disadvantages of the employed ligands are discussed and it…

Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton…

Introduction Endotoxins are lipo poly saccharides present in the cell wall of most Gram-negative bacteria, and are frequently present as contaminants in protein solutions purified…

The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit is an efficient, quantitative endpoint assay for the detection of gram-negative bacterial endotoxins. Bacterial endotoxin catalyzes…