Tue. Dec 10th, 2019

G-Bioscience The Column PROTEIN- Concentrate Kit

2 min read
international center for biotechnology information

international center for biotechnology information

Introduction:

Many precipitation techniques are currently used for concentrating dilute protein solutions. Some of the precipitation techniques are not suitable for concentrating large volumes of dilute protein solutions. Furthermore, many fragile and enzymatic proteins may lose their biological activity when concentrated by acid precipitation techniques. Conventional chromatography techniques are only useful for concentrating dilute protein solution when the binding & elution characteristics of the protein are established. Yet another limitation of the conventional chromatography is that they require large elution volumes. The Column PROTEIN- Concentrate Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in low salt buffer between pH 2-12 (capacity ~ 0.5mg protein/ml Protein Binding Resin). The immobilized protein is spin-eluted in a small volume of specifically formulated elution buffer, giving several fold effective concentration. This kit is suitable for concentration of a total of 4mg protein in either single or multiple procedures.

The Column PROTEIN- Concentrate Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in low salt buffer between pH 2-12 (capacity ~ 0.5mg protein/ml Protein Binding Resin). The immobilized protein is spin-eluted in a small volume of specifically formulated elution buffer, giving several fold effective concentration. This kit is suitable for concentration of a total of 4mg protein in either single or multiple procedures.

Leave a Reply

Your email address will not be published. Required fields are marked *