GE Healthcare Membrane Protein Purification Kit.

Membrane Protein Purification Kit. Efficient detergent screening of histidine-tagged membrane proteins. Small aliquots of cell membranes are solubilized in different detergents followed by rapid purification using His Mag Sepharose™ Ni. The purification step is performed directly after solubilization using the same detergent. Analysis and evaluation can be performed by a number of methods such as Western blot, gel filtration, or light scattering.

Membrane Protein Purification Kit (Fig 1) is designed for efficient detergent screening of histidine-tagged membrane proteins. Small aliquots of cell membranes are solubilized in different detergents followed by rapid purification using His Mag Sepharose™ Ni. The purification step is performed directly after solubilization using the same detergent. Analysis and evaluation can be performed by a number of methods such as Western blot, gel filtration, or light scattering.Key benefits:

• Easy solubilization and purification of membrane proteins

• Rapid detergent screening in the convenient magnetic bead format

• Simultaneous evaluation of solubilization and purification efficiency

• Ready-to-use detergent solutions

• Easy-to-follow protocol

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