GE-HEALTHCARE PreDictor plates for High-throughput process development (HTPD)

protein purification strategy

Disposable 96-well filter plates prefilled with GE Healthcare BioProcess™ chromatography media (Capto™ Q, S, DEAE, MMC, Adhere; Q and SP Sepharose™; MabSelect™, SuRe™ and Xtra™; AIEX and CIEX screening plate. Support HTPD by allowing parallel screening of chromatographic conditions. They can be used in automated workflows using robotic systems, or can be operated manually using multi-channel pipettes

Introduction:

PreDictor™ plates are disposable 96-well filter plates prefilled with GE Healthcare BioProcess™ chromatography media, see Table 1. PreDictor plates support high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions. They can be used in automated workflows using robotic systems, or can be operated manually using multi-channel pipettes. Each well in a PreDictor plate is prefilled with a defined amount of chromatography medium. The choice of PreDictor plate depends on the type of application, see Table 2 and Section 2.2 for details. As a result of parallel screening of chromatographic conditions, a large number of experimental conditions may be evaluated simultaneously. This allows screening of a large experimental space to identify the subspace that is the most relevant with respect to one or several defined responses. All chromatography media used in PreDictor plates are available in prepacked columns and in bulk packs. This means that once the experimental subspace has been found, optimization and scale-up can easily be done on columns using ÄKTAdesign™ systems.

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