For chromatographic media a simple and efficient regeneration method is as important as high protein binding capacity and high resolution. Regeneration should be performed directly in the column to avoid new packing. This method is called”cleaning-in-place” (CIP).
The column geometry which provides a good distribution of cleaning solution is essential for complete removal of contamination. Especially in the region around the adaptor sealings, dead space shave to be avoided because of their susceptibility to contamination with microrganisms.