MILLIPORE Detergent Removal with Amicon® Microcon® and Centricon® Centrifugal Devices

bio protein

Amicon Microcon and Centri concentrifugal devices are efficient laboratory tools for removing small molecules from solutions of proteins ornucleic acids. Often, the molecule tobe removed is one of a number ofcommonly used protein solubilizing agents, or detergents. The chemical nature of most deter-gents allows for micelle formation above a critical concentration limit(Critical Micelle Concentration,CMC). Micelle formation results inaggregation of the detergent andleads to gross changes in molecular structure. This affects the amount ofthe detergent that can be removed from a solution by Microcon andCentricon devices with specific molecular weight cut-off (MWCO)membranes.

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