Mon. Dec 9th, 2019

NORGEN ProteoSpin™ Detergent Clean-Up Kit.

3 min read
bio protein

bio protein

ProteoSpin™ Detergent Clean-Up Micro Kit – Product Insert Product # 10200, 10500

The ProteoSpin™ Detergent Clean-Up Micro Kit provides a fast and simple procedure for the removal of SDS, Triton® X-100 and other detergents from protein samples. Detergents are extensively used to prepare protein samples; however, these detergents must often be removed prior to downstream analysis because of their undesirable effects. These include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. The ProteoSpin™ Detergent Clean-Up Micro Kit can remove greater than 95% of detergents from protein samples while maintaining high protein recovery. The kit is able to remove all types of detergents including ionic, non-ionic and zwitterionic detergents. It is designed to remove detergents in protein solutions either in their free form or bound form, as when complexed with the protein. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The purified protein can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, proteolytic digestion, isoelectric focusing, x-ray crystallography, and NMR spectroscopy. The ProteoSpin™ Detergent Clean-Up Micro Kit contains solutions for the processing of both acidic and basic protein samples. Each spin column is able to process small detergent-containing samples from 2 – 50 μg of protein. Detergents including SDS, Triton® X-100, CHAPS, NP-40 and Tween 20 can be removed using the kit, with protein recoveries of 80 – 95% for most proteins. The kit has a shelf life of at least 1 year when stored as suggested.

ProteoSpin™ Detergent Clean-Up Maxi Kit – Product Insert Product # 17100

The ProteoSpin™ Detergent Clean-Up Kit provides a fast and simple procedure for the removal of SDS, Triton® X-100 and other detergents from protein samples. Detergents are extensively used to prepare protein samples; however, these detergents must often be removed prior to downstream analysis because of their undesirable effects. These include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. The ProteoSpin™ Detergent Clean-Up Maxi Kit can remove greater than 95% of detergents from protein samples while maintaining high protein recovery. The kit is able to remove all types of detergents including ionic, non-ionic and zwitterionic detergents. It is designed to remove detergents in protein solutions either in their free form or bound form, as when complexed with the protein. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The purified protein can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, proteolytic digestion, isoelectric focusing, x-ray crystallography, and NMR spectroscopy. The ProteoSpin™ Detergent Clean-Up Maxi Kit contains solutions for the processing of both acidic and basic protein samples. Each spin column is able to process detergent-containing samples from 0.25 – 8 mg of protein. Detergents including SDS, Triton® X-100, CHAPS, NP-40 and Tween 20 can be removed using the kit, with protein recoveries of 80 – 95% for most proteins. The kit has a shelf life of at least 1 year when stored as suggested.


Leave a Reply

Your email address will not be published. Required fields are marked *