ProteoExtract® Native Membrane Protein Extraction Kit (M-PEK) is designed for the isolation of native membrane proteins from mammalian cells and tissue. The membrane protein fraction is separated in less than two hours without the need for ultracentrifugation or incubation of samples at elevated temperatures
Membrane proteins represent only one-third of the proteins encoded by the human genome, but they represent more than two-thirds of the known protein targets for drugs. Therefore, approaches to prepare and characterize membrane proteins are of significant interest for drug discovery. However, due to their hydrophobic nature, membrane proteins are more difficult to analyze than soluble proteins. In addition to the intrinsic difficulty of solubilization, membrane proteins are challenging because of their general low abundance. Thus, the challenges for sample preparation of membrane proteins are effective solubilization and selective enrichment, ideally keeping proteins in a non-denatured state. The ProteoExtract™ Native Membrane Protein Extraction Kit (M-PEK) is designed for the isolation of native membrane proteins from mammalian cells and tissue. It has been developed for convenient high yield membrane protein extraction, providing 3-5 fold enrichment of integral membrane and membrane-associated proteins using non-denaturing conditions. The kit belongs to a range of related kits from Calbiochem all aimed at reducing sample complexity in order to improve the probability of identifying low abundance proteins (see Section 9: Related Products). M-PEK is easy to use and allows for a fast and robust two-step extraction of membrane proteins from mammalian samples. Optimized protocols are provided for adherent tissue culture cells, suspension grown tissue culture cells, frozen cell pellets and tissue.