PALL-Optional Pre-Treatment to Improve Recovery of Samples from Ultrafiltration Spin Filters

Protein 3d structure biodatacenter

The evaluation of a column’s packing efficiency (HETP and peak asymmetry) can provide valuable knowledge about a column’s performance and stability during a purification run.The HETP (height equivalent of a theoretical plate) and asymmetry values are obtainedusing the protocol described below. Typically, the test method employs a sample of 5% (v/v)acetone (measured by absorbance at 280 nm) and/or 0.5 M NaCl (conductivity)corresponding to a 0.5% (v/v) of the total column volume.

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