PALL Protein Sample Preparation and Analysis Application Manual

Sigma Protease Iranian Bio Data Center

Complexity reduction in samples is an important step in facilitating access to the low abundant proteins of interest for disease research and diagnostics. This process for human serum and plasma frequently includes the depletion of high abundant proteins such as albumin and IgG in combination with other fractionation techniques prior to 2D gel electrophoresis or LC-MS/MS detection.Pall currently offers several solutions for abundant protein separation or removal. Options include affinity chromatography resins as well as complete kits for removal of multiple abundant proteins (albumin, IgG) in one step.Enchant™ Protein Separation Kits rapidly deplete abundant proteins and unmask low abundant biomarkers from serum and plasma samples. Alternatively, these kits can be used to collect the abundant protein fractions for further analysis

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