Tue. Dec 10th, 2019

PROMEGA Canine Pancreatic Microsomal Membranes.

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Promega

Promega

Canine Pancreatic Microsomal Membranes. To study cotranslational and initial post-translational processing of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation, and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes

Microsomal vesicles are used to study cotranslational and initial post-translationalprocessing of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation, and core glycosylation can be examined by thetranslation of the appropriate mRNA in vitro in the presence of these microsomalmembranes. In addition, processing and glycosylation events may be studied by thetranscription/translation of the appropriate DNA in the TNT®Lysate Systems(a,b,c,d)when used with microsomal membranes. To assure consistent performance withminimal translational inhibition and background, microsomes have been isolated freefrom contaminating membrane fractions and stripped of endogenous membrane-bound ribosomes and mRNA (1). Membrane preparations are assayed for both sig-nal peptidase and core glycosylation activities using two different control mRNAs.The two control mRNAs supplied with this system are the precursor for β-lactamase (or ampicillin resistance gene product) from E. coliand the precursor for α-matingfactor (or α-factor gene product) from S. cerevisiae.Potential applications of the Canine Pancreatic Microsomal Membranes(d)include:•Signal peptide cleavage examination•Membrane insertion and/or translocation analysis•Core glycosylation analysis

PROMEGA Canine Pancr Microsomal Membrane Manual PDF

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