QIAGEN: EasyXpress™ Insect Cell Protein Synthesis handbook.

Qiagen

EasyXpress™ Insect Cell Protein Synthesis Handbook. EasyXpress Protein Synthesis Insect Kit. EasyXpress pIX4.0 Vector. For in vitro synthesis of eukaryotic proteins (including membrane proteins) with posttranslational modifications using insect cell lysates In vitro translation is a widely used tool for the production of recombinant proteins. Proteins produced by in vitro translation can be used for a wide variety of downstream applications, including activity assays, protein–protein interaction studies, and the expression and analysis of open reading frames and expression

constructs. A broad range of eukaryotic proteins require posttranslational modifications such as phosphorylation, glycosylation, or signal peptide cleavage to display full functional activity. Eukaryotic in vitro translation systems provide the possibility to synthesize eukaryotic proteins with posttranslational modifications and are of special importance for expression and analysis of human proteins with native structure and function. To address these needs, QIAGEN has developed the EasyXpress Protein Synthesis Insect Kit, a new eukaryotic in vitro translation system fo r expression of eukaryotic
proteins including membrane protein with posttranslational modifications. In contrast to many rabbit reticulocyte lysate (RRL) -based systems, the insect-cell extract does not require any additives to display full functionality. In addition, the EasyXpress pIX4.0 Vector has been developed for generation of optimal
expression templates to be used with the EasyXpress Protein Synthesis Insect Kit.
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