SUMOpro-3 ® Gene Fusion Technology

SUMOpro ® Gene Fusion Technology

Download SUMOpro-3 ® Gene Fusion Technology Catalogue PDF

The SUMOpro-3 Protein and Peptide Expression Kit maximizes the yield of soluble, untagged, functional proteins with desired N-termini. The system uses an active and specific SUMO Protease 2 that recognizes the natural tertiary structure of the SUMO (HuSUMO-3) tag, generating the desired N-terminus and never cleaving within your protein of interest. By linking a 6XHis epitope to both the SUMO-3 fusion tag and SUMO Protease 2, the SUMOpro-3 system allows efficient and rapid purification of untagged protein using Ni-NTA chromatography. The SUMOpro-3 kit is recommended for expression and purification of proteins that contain many hydrophobic residues at or around the N-terminus.

Leave a Reply

Your email address will not be published. Required fields are marked *