Chemical COmpounds Iranian Bio Data Center
Posted in Detergents

CALBIOCHEM Biological Detergents. Guide for solubilization of membrane proteins and selecting tools for detergent removal

Biological membranes, composed of complex assemblies of lipids and proteins, serve as physi-cal barriers in the cell and are sites for many cellular signaling events….

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Posted in Detergents

maltose–neopentyl glycol (mn G) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

Integral membrane proteins (IMPs) play crucial roles in many aspects of biology by mediating the transfer of material and sig-nals between cells and their environment….

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Posted in Detergents

ANATRACE Neopentyl Glycol (NG) class detergents

Neopentyl Glycol (NG) class detergents are revolutionary new amphiphiles which have already shown great utility in membrane protein studies. NG class detergents are a more…

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Posted in Detergents

ANATRACE Amphipol A8-35

Amphipol A8-35  Amphipathic surfactant for maintaining solubilized membrane proteins in detergent-free solutions. Serve as stabilizers of membrane proteins in aqueous solutions. Amphipols can substitute out…

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Iranian Bio Data Center
Posted in Detergents

ANATRACE Structure of Detergents

Chemical structure of Detergents used in biotechnology experiments. useful reference for understanding chemical interactions and structures through laboratory.

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International center for biotechnology information
Posted in Detergents

ANATRACE How to purify a membrane protein-Technical bulletin

1- First Step! Look up other purifications from similar sources and similar membranes.Different tissues, organisms and membrane types sometimes require differ-ent detergents and conditions. e.g….

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international center for biotechnology information
Posted in Endotoxin removal and Endotoxin test

Broad application and optimization of a single wash-step for integrated endotoxin depletion during protein purification

Abstract: Endotoxins contaminate proteins that are produced inE. coli.High levels of endotoxins can influence cellular assays and cause severe adverse effects when administered to humans….

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international center for biotechnology information
Posted in Uncategorized

Efficient production of recombinant IL-21 proteins for pre-clinical studies by a two-step dilution refolding method

Introduction Interleukin 21 (IL-21) is a recently discovered type I cytokine and classified in the family, which includes IL-2, IL-4, IL-7, IL-9, IL-13 andIL-15, all…

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international center for biotechnology information11
Posted in Endotoxin removal and Endotoxin test

Simultaneous metal chelate affinity purification and endotoxin clearance of recombinant antibody fragments

Abstract: Endotoxins are frequent contaminants of recombinant proteins produced inEscherichia coli. Due to their adverse effects, endotoxins have to be removed from recombinant proteins prior…

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international center for biotechnology information
Posted in Endotoxin removal and Endotoxin test

Review: Endotoxin removal by affinity sorbents

Introduction: Affinity sorbents and detoxification strategies are described to remove different amounts of endotoxin. Advantages and disadvantages of the employed ligands are discussed and it…

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Endotoxin removal
Posted in Endotoxin removal and Endotoxin test

Removal of endotoxin from protein solutions by phase separation using Triton X-114

Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton…

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international center for biotechnology information
Posted in Endotoxin removal and Endotoxin test

Removal of endotoxin from monoclonal antibodies using Vivapure centrifugal ion exchange membrane devices

Introduction Endotoxins are lipo poly saccharides present in the cell wall of most Gram-negative bacteria, and are frequently present as contaminants in protein solutions purified…

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ENDOTOXIN iranian bio data center
Posted in Endotoxin removal and Endotoxin test

THERMO: The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit

The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit is an efficient, quantitative endpoint assay for the detection of gram-negative bacterial endotoxins. Bacterial endotoxin catalyzes…

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Iranian Bio Data Center
Posted in Endotoxin removal and Endotoxin test

THERMO: Pierce High-Capacity Endotoxin Removal Resin.

The Thermo Scientific Pierce High Capacity Endotoxin Removal Resin contains porous cellulose beads that have been surface modified with covalently attached, modified ε-poly-L-lysine. Modified polylysine…

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Protein Inhibitor
Posted in Endotoxin removal and Endotoxin test

THERMO: Detoxi-Gel Endotoxin Removing gel

The Detoxi-Gel™ Endotoxin Removing Gel uses immobilized polymixin B to bind and remove pyrogens from solution. Thepolymixins are a family of antibiotics that contain a…

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