The iCON™ Concentrators are disposable ultrafiltration centrifugal devices for concentration and diafiltration/bufferexchange of biological samples such as enzymes, antibodies or DNA. These concentrators provide exceptional speed, proteinrecovery and ease-of-use in a one-step procedure. The unique conical design and high-performance membranes maximizesurface area while minimizing protein polarization and adsorption at the membrane surface, providing reliable and consistentresults. Typical protein recovery for proteins larger than the membrane molecular weight cut-off is >90% and greater than150-fold concentration may be achieved in less than 30 minutes for the 9K MWCO and less than 15 minutes for the 20KMWCO. The iCON™ Concentrators are compatible with most swinging-bucket (preferred) or fixed-angled rotors.
•The iCON™ Concentrators can be used effectively at a relative centrifugal force of 2,000-9,000 x g; the recommendedspeeds are 2,500-4,500 x g for swinging-bucket rotors and 5,000-7,000 x g for fixed-angle rotors.Note: The maximum centrifugal force for many swinging bucket rotors is 3,000-4,000 x g. Do not exceed the maximumrecommended centrifugal force of the rotor being used.
•Ensure rotor and carriers are compatible with centrifugal device and allow proper clearance for swinging-bucket rotorsand/or centrifuge lid. Ensure devices and rotors are properly balanced before centrifugation.
•Sample volume, centrifugal force, temperature, sample concentration and viscosity affect filtration rate. Optimizecentrifugal time for each application.Using a fixed-angle rotor at 6,000 x g and 22°C, 4 ml of a 0.2 mg/ml proteinsample will typically decrease in volume by 20- to 25- fold in 20 minutes for the 9K MWCO or 25- to 30-fold in 8minutes for the 20K MWCO.
•The dead-stop volume of the 7 ml devices is approximately 10 μl when processing samples <6 ml or with decanting ofexcess filtrate from samples >6 ml, which allows for concentration factors in excess of 500-fold.Note: Precipitation may occur at high concentration factors for some proteins. Maximum concentration factor isdependent on the specific protein, starting concentration and buffer system. Unless the stability of a protein has beendetermined, avoid concentrating to dead-stop. Pre-filling the collection tube with a known volume of buffer can controlthe dead-stop volume and final concentration factor for a sample (see Appendix A).
•The concentrators contain a high-performance regenerated cellulose membrane rated for retaining molecules greater thanthe indicated MWCO (>90% recovery). Reduced recovery may occur with molecules that are less than the indicatedMWCO. Recovery will vary depending on the specific protein or starting concentration.
•Avoid scraping the membrane surface when adding or recovering sample.
•Do not autoclave iCON™ Concentrators.