The Thermo Scientific Pierce High Capacity Endotoxin Removal Resin contains porous cellulose beads that have been surface modified with covalently attached, modified ε-poly-L-lysine. Modified polylysine has a high affinity for endotoxinswith the affinity ligand eliminating toxicity associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers. The binding capacity of 2,000,000 Endotoxin Units (EU)/mL allows endotoxin levels to be reduced by 99% in samples containing 10,000EU/mL; typical protein samples processed with the resin have a final endotoxin concentration below 5EU/mL. The resin is offered in a slurry format for custom packing of endotoxin removal columns for gravity flow or continuous flow (10-15 mL/hour) applications or a spin column format for the fast, single-use batch method. Removal of endotoxins from differing volumes of biological samples can be done in ≥ 1 hour (see Table 1).Endotoxins consist of lipopolysaccharides (LPS), which are biologically active, structural components of the outer cell membrane of all gram-negative bacteria. Small amounts of endotoxin in recombinant protein preparations can cause side effects, including endotoxin shock, tissue injury and death in host organisms; therefore, it is essential to remove endotoxins from drugs, injectables and other biological products.