THERMOFISHER: Nuclei Enrichment Kit for Tissue


The Nuclei Enrichment Kit for Tissue enables isolation of an enriched sample containing intact nuclei from 400-500 mg of either hard or soft tissue. The kit contains OptiPrep™ Cell Separation Media and optimized buffers along with detailed instructions for the enrichment of nuclei with minimal contamination from golgi bodies and endoplasmic reticulum. The procedure provided is a guideline and some optimization may be required to ensure optimal results for each tissue type. The isolated, intact nuclear sample contains all the nuclear proteins, genetic material and other intact nuclear structures, allowing downstream applications such as enzymatic assays, electron microscopy, Western blotting and 2D/MS analysis

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